mnng hos os cells  (ATCC)

Journal: Journal of Nanobiotechnology

Article Title: Cargo-eliminated osteosarcoma-derived small extracellular vesicles mediating competitive cellular uptake for inhibiting pulmonary metastasis of osteosarcoma

doi: 10.1186/s12951-024-02636-9

Figure Lengend Snippet: Characterization of OS-sEVs and CE-sEVs. (A) Western blot analysis of sEVs characteristic markers (CD9, CD63, and TSG101) and non-sEVs marker GM130 in MNNG cells and OS-sEV. (B) Representative TEM images of OS-sEVs and CE-sEVs. Scale bar: 100 nm. (C) Particle size distribution of OS-sEVs and CE-sEVs. (D) Quantification of the mean protein concentration per particle of OS-sEVs and CE-sEVs ( n = 3). (E) Sliver staining image of total proteins in OS-sEVs (1 × 10 10 particles) and CE-sEVs (1 × 10 10 particles) and (F) the quantification of the relative protein content of OS-sEVs and CE-sEVs ( n = 3). (G) RNA enrichment analysis depicted in FU per nt of total RNA contents in OS-sEVs (1 × 10 10 particles) and CE-sEVs (1 × 10 10 particles). (H) Total RNA contents in OS-sEVs (1 × 10 10 particles) and CE-sEVs (1 × 10 10 particles) by SYTO™ RNA staining ( n = 3). *** P < 0.001; # P < 0.0001

Article Snippet: The human OS cell line MNNG/HOS (MNNG) and the human osteoblast cell line hFOB1.19 were purchased from the American Type Culture Collection (ATCC).

Techniques: Western Blot, Marker, Protein Concentration, Staining

Journal: Journal of Nanobiotechnology

Article Title: Cargo-eliminated osteosarcoma-derived small extracellular vesicles mediating competitive cellular uptake for inhibiting pulmonary metastasis of osteosarcoma

doi: 10.1186/s12951-024-02636-9

Figure Lengend Snippet: CE-sEVs exhibit no tumor-promoting ability. (A) CCK-8 assay detects the proliferation of MNNG cells after treated with OS-sEVs and CE-sEVs, the results confirm that OS-sEVs promoted OS cell proliferation, while CE-sEVs did not have such effect ( n = 3). (B) Transwell assay detects the migration of MNNG cells after treated with OS-sEVs and CE-sEVs, the results confirm that OS-sEVs promoted OS cell migration, while CE-sEVs did not have such effect ( n = 3). (C) Tumor resection images after intravenous intervention with OS-sEVs ( n = 5), CE-sEVs ( n = 5), and control ( n = 5) in the nude mouse subcutaneous tumor model, administered three times per week. (D) Quantitation of tumor weight (mean ± SD). (E) Quantitation of tumor volume (mean ± SD). ns P > 0.05; * P < 0.05; *** P < 0.001

Article Snippet: The human OS cell line MNNG/HOS (MNNG) and the human osteoblast cell line hFOB1.19 were purchased from the American Type Culture Collection (ATCC).

Techniques: CCK-8 Assay, Transwell Assay, Migration, Control, Quantitation Assay

Journal: Journal of Nanobiotechnology

Article Title: Cargo-eliminated osteosarcoma-derived small extracellular vesicles mediating competitive cellular uptake for inhibiting pulmonary metastasis of osteosarcoma

doi: 10.1186/s12951-024-02636-9

Figure Lengend Snippet: CE-sEVs efficiently suppressed the pulmonary metastasis of OS. (A) Schematic representation of the experimental metastasis model of OS. The mice were divided into three groups and pretreated with blank (control group), OS-sEVs (OS-sEVs group), OS-sEVs + CE-sEVs (CE-sEVs group) every three days. Then, MNNG cells were intravenous injection on day 12, mice were euthanized on day 28, and lungs were excised for observation of metastasis using BLI. (B) Representative ex vivo BLI of the lungs in experimental metastasis model, and the pulmonary metastasis of MNNG cells were calculated based on the lung’s FI value. (C) Quantification of lung’s FI in experimental metastasis model ( n = 4/5). (D) Schematic representation of the spontaneous metastasis model of OS. The mice received MNNG cells inoculation into the tibia on day 0, and then divided into three groups: the control group (administered with blank), the PBS group (administered with PBS), and the CE-sEVs group (administered with CE-sEVs). Thrice-weekly interventions were performed. At the end of week 4, the mice were euthanized, and lungs were excised for observation of metastasis using BLI. (E) Representative ex vivo BLI of the primary tumors and lungs in spontaneous metastasis model, and the pulmonary metastasis of MNNG cells were calculated based on the lung’s FI value. (F) Quantification of lung’s FI in spontaneous metastasis model by BLI ( n = 4/5). (G) Kaplan-Meier analysis of survival time in mice with spontaneous OS metastasis model ( n = 8). ns P > 0.05; * P < 0.05; ** P < 0.01

Article Snippet: The human OS cell line MNNG/HOS (MNNG) and the human osteoblast cell line hFOB1.19 were purchased from the American Type Culture Collection (ATCC).

Techniques: Control, Injection, Ex Vivo